Analysing Gene Expression: A Handbook of Methods Possibilities and Pitfalls provides a broad view of the molecular biological and biochemical methods which are now available to analyse the expression of mRNAs and proteins in different cells and tissues.

Despite the broadness of the apporach, sufficient detail is provided to enable the reader to grasp the inherent principle of each method. Attention is especially paid to the relative advantages and disadvantages of each method. Information about newer methods such as DNA-chips, digital expression pattern display, and total analysis of gene expression is also included.

This two volume set is an indispensable reference tool for professional scientists in biochemistry, molecular biology, and medicine. It will also be of particular interest to commercial enterprises in the biotechnology field including companies involved in pharmaceuticals, diagnostics and therapeutics.

Contents

Volume 1

1. Basic concepts of gene expression
2. Sample preparation and supplementary tools
3. Methods for analysing mRNA expression

Volume 2

4. High-throughput and industrial methods for mRNA expression analysis
5. Protein expression analysis
6. Methods for mRNA and protein expression analysis in situ and in vivo
7. Computational methods and bioinformatic tools

Index

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