Aquaculture Genome Technologies bridges the gap between basic genomic technology and how to systematically apply this information to aquaculture genomes.
This book provides a tour through the aquaculture genomics process providing basic principles and detailed methodologies as to how to use genome technologies to study the genomes of aquaculture species.
Contents
- Concept of genome and genomics
Part 1: Marking Genomes
- Restriction fragment length polymorphism (RFLP)
- Randomly amplified polymorphic DNA (RAPD)
- Amplified fragment length polymorphism (AFLP)
- Microsatellite markers and assessment of marker utility
- Single nucleotide polymorphism (SNP)
- Allozyme and mitochondrial markers
- Individual-based genotype methods in aquaculture
- Application of DNA markers for population genetic analysis
Part 2: Mapping Genomes
- Linkage mapping in aquaculture species
- Detection and analysis of quantitative trait loci (QTLs) for economic traits in aquatic species
- Marker Assisted Selection for Aquaculture Species
- Construction of large-insert bacterial clone libraries and their applications
- Bacterial artificial chromosome libraries and BAC-based physical mapping of aquaculture genomes
- Physical characterization of aquaculture genomes through BAC end sequencing
- Genomescape: characterizing the repeat structure of the genome
- Genomic analyses using fluorescence in situ hybridization
- Radiation hybrid mapping in aquatic species
- Comparative Genomics and Positional Cloning
Part 3. Analysis of Genome Expression and Function
- Transcriptome characterization through the analysis of expressed sequence
- Microarray fundamentals: Basic principles and application in aquaculture
- Salmonid DNA microarrays and other tools for functional genomics research
- Computational challenges for the analysis of large datasets related to aquatic environmental genomics
- Functional genomics
Part 4. Preparing for Genome Sequencing
- DNA sequencing technologies
- Sequencing the genome
- Bioinformatics
Part 5. Dealing with the Daunting Genomes of Aquaculture Species
- Dealing with duplicated genomes of teleosts
- Bivalve genomics: complications, challenges, and future perspectives
Index