Arthritis Research is a compendium of data pertinent ot the methods and protocols that have contributed to recent advances in molecular medicine in general, and to the molecular basis of rheumatic disease in particular.

Arthritis Research, Volume 1 discusses:

• synovial joint morphology
• histopathology
• immunohistochemistry
• cartilage matrix and bone biology
• cell trafficking
• migration and invasion

Contents

Synovial Joint Morphology, Histopathology, and Immunohistochemistry

1. Imaging Inflamed Synovial Joints
2. Arthroscopy as a Research Tool: A Review
3. Immunohistochemistry fo the Inflamed Synovium
4. In Situ Hybridization of Synovial Tissue
5. Subtractive Hybridization
6. Laser Capture as a Tool for Anaylsis of Gene Expression in Inflamed Synovium
7. Preparation of Mononuclear Cells from Synovial Tissue
8. Quantitative Image Analysis of Synovial Tissue

Cartilage Matrix and Bone Biology

9. Cartilage Histomorphometry
10. Image Analysis of Aggrecan Degradation in Articular Cartilage with Formalin-Fixed Samples
11. In Situ Detection of Cell Death in Articular Cartilage
12. Measurement of Glycosaminoglycan Release from Cartilage Explants
13. Assessment of Collagenase Activity in Cartilage
14. Assessment of Gelatinase Expression and Activity in Articular Cartilage
15. Anaysis of MT1-MMP Activity in Cells
16. Analysis of TIMP Expression and Activity
17. Bone Histomorphology in Arthritis Models
18. Generation of Osteoclasts in Vitro, and Assay of Osteoclast Activity

Cell Trafficking, Migration, and Invasion

19. Isolaton and Analysis of Large and Small Vessel Endothelial Cells
20. Analysis of Flow-based Adhesion In Vitro
21. Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy
22. Angiogenesis in Arthritis: Methodological and Analytical Details
23. Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity
24. Acquisition, Culture, and Phenotyping of Synovial Fibroblasts
25. Genotyping of Synovial Fibroblasts: cDNA Array in Combination with RAP-PCR in Arthritis
26. Gene Transfer to Synovial Fibroblast: Methods and Evaluation in the SCID Mouse Model
27. In Vitro Matrigel Fibroblast Invasion Assay
28. Culture and Analysis of Circulating Fibrocytes

Index

Arthritis Research: Methods and Protocols Vol. 1 accompanies
Arthritis Research: Methods and Protocols Vol. 2

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