Arthritis Research is a compendium of data pertinent ot the methods and protocols that have contributed to recent advances in molecular medicine in general, and to the molecular basis of rheumatic disease in particular.

Arthritis Research, Volume 2 covers:

• immunobiolgy and T cells
• animal models of arthritis
• applications of new technologies to define novel therapeutic targets

Contents

Immunobiology

1. Phenotypic Analysis of B-Cells and Plasma Cells
2. Detection of Antigen Specific B-Cells in Tissues
3. Single Cell Analysis of Synovial Tissue B-Cells
4. Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules
5. Analysis of Antigen Reactive T-Cells
6. Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells
7. Analysis of Th1/Th2 T-Cell Subsets
8. Analysis of the T-Cell Receptor Repertoire of Synovial T-Cells
9. The Assessment of T-Cell Apoptosis in Synovial Fluid
10. Assays of T-Cell Contact Dependent Monocyte-Macrophage Functions
11. Phenotypic and Functional Analysis of Synovial Natural Killer Cells
12. Identification and Isolation of Synovial Dendritic Cells

Animal Models of Arthritis

13. The Use of Animal Models for Rheumatoid Arthritis
14. Collagen-Induced Arthritis in Mice
15. Collagen-Induced Arthristis in Rats
16. Collagen Antibody Induced Arthritis
17. Arthritis Induced with Minor Cartilage Proteins
18. Murine Antigen-Induced Arthritis
19. Pristane-Induced Arthritis in the Rat
20. The K/BxN Mouse Model of Inflammatory Arthritis: Theory and Practice
21. Analysis of Arthritic Lesions in the Del1 Mouse: A Model for Osteoarthritis

Application of New Technologies to Define Novel Therapeutic Targets

22. Gene Expression Profiling in Rheumatology
23. Differential Display Reverse Transcription-Polymerase Chain Reaction to Identify Novel Biomolecules in Arthritis Research
24. Two-Dimensional Electrophoresis of Proteins Secreted from Articular Cartilage
25. Mapping Lymphocyte Plasma Membrane Proteins: A Proteomic Approach
26. In Vivo Phage Display Selection in the Human/SCID Mouse Chimera Model for Defining Synovial Specific Determinants
27. Adenoviral Targeting of Signal Transduction Pathways in Synovial Cell Cultures

Index

Arthritis Research: Methods and Protocols Vol. 2 accompanies
Arthritis Research: Methods and Protocols Vol. 1

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