• Presents in a step-by-step fashion those techniques used by immunologists to produce antibodies
• Addresses immunizations; polyclonal and monoclonal antibodies; and purification, characterization, and storage

Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies.

Antibodies described include polyclonal and monoclonal and those made by the phage display. This volume focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.

Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.

Contents

1. Starting Out
2. Animal Welfare and Regulatory Issues
3. Antigens
4. Adjuvants
5. Polyclonal Antibodies
6. Monoclonal Antibody Protection
7. Screening Techniques
8. Antibody Production by Ascites Growth
9. Growth of Cell Lines in Bioreactors
10. Phage Display
11. Antibody Purification Methods
12. Molecular Characterization of Immunoglobulin Genes
13. Antibody Storage
14. Chemically Modifying Antibodies
15. Western Blotting
16. Immunohistochemical Methods

click here to see books • videos • cd-roms of related interest