Bioanalytical Chemistry
by Susan R. Mikkelson
and Eduardo Cortón

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry!

Featuring many of the techniques used by biochemists and molecular biologists, Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. The end result is an authoritative primer to this expanding field–one that will prove invaluable for advanced undergraduate chemistry and biochemistry students, bioanalytical chemistry graduate students, and practitioners. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples.

Coverage includes:

• Spectroscopic methods for matrix characterization
• Structural and functional properties of antibodies
• Principles of electrophoresis
• Centrifugation methods
• Mass spectrometry of biomolecules
• Quantitation of enzymes and their substrates
• Design and implementation of enzyme assays
• Isoelectric focussing
• Chromatography of biomolecules
• Validation of new bioanalytical methods
• And much more!

This indispensable guide features end-of-chapter problems and selected answers in the appendix, designed to help students assess their knowledge of each topic. Each chapter also includes suggestions for further reading, and mathematical derivations have been kept to a minimum throughout.

More than any other reference, Bioanalytical Chemistry captures the interdisciplinary scope of this emerging field–and uniquely prepares students for collaborative scientific work and research in industrial, government, and academic laboratories.

Contents

1. Spectroscopic Methods for Matrix Characterization
2. Enzymes
3. Quantitation of Enzymes and Their Substrates
4. Immobilized Enzymes
5. Antibodies
6. Quantitative Immunoassays with Labels
7. Biosensors
8. Directed Evolution for the Design of Macromolecular Bioassay Reagents
9. Principles of Electrophoresis
10. Applications of Zone Electrophoresis
11. Isoelectric Focusing
12. Capillary Electrophoresis
13. Centrifugation Methods
14. Chromatography of Biomolecules
15. Mass Spectrometry of Biomolecules
16. Validation of New Bioanalytical Methods

Answers to Problems
Index

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