by M.A. Vijayalakshmi

The field of bioseparation, and biochromatography in particular, is advancing very rapidly as our knowledge of the properties of molecules and atomic forces increases. This volume covers the basic principles of biochromatography in detail. It assesses different techniques and includes a large number of applications, providing the reader with a multidisciplinary perspective that gives the insight to master the many chromatographic methods.

Biochromatography: Theory and Practice is a valuable tool for graduate and research scientists, technicians, engineers and teachers in a range of fields including biochemistry, biotechnology, biorecognition and chromatography.

Contents

1. Upstream and Downstream Steps in Biochemistry
2. Gel Filtration
3. Ion Exchange Interaction Chromatography
4. Hydrophobic ( Interaction ) Chromatography of Proteins
5. Conformational Behaviour of Polypeptides and Proteins in Reversed Phase and Lipophilic Environments
6. Affinity Chromatography
7. Dye Ligand Affinity Chromatography
8. Immobilized Synthetic Dyes in Affinity Chromatography
9. Immobilized Histidine Ligand Affinity Chromatography
10. Immobilized Metal-Ion Affinity Chromatography: From Phenomenological Hallmarks to Structure-Based Molecular Insights
11. Thiophilic Interaction Chromatography
12. Miscellaneous Methods in Affinity Chromatography
    • Part 1: Boronic Acids as Selective Ligands for Affinity Chromatography
    • Part 2: Shielded Affinity Chromatography in Packed Bed and Expanded Bed Mode
13. Glycobiology and Biochromatography
14. Capillary Electrokinetic Chromatography
15. Imprintec Polymers as Tailor-Made Stationary Phases for Affinity Separation
16. Computer-aided Simulation of Biochromatography
17. Industrial Biochromatography: Engineering Aspects
18. Validation Aspects in Biochromatography
19. Biochromatography and Biomedical Applications

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