The present selection of 12 inspiring experiments has been carefully selected to cover the most commonly used laboratory techniques for the synthesis and analysis of bioactive compounds. Together, they provide a solid introduction to the skills and techniques that are required for the successful study of Chemical Biology.

Learn how to:

• Synthesize a peptide by solid phase synthesis
• Modify a protein by covalent attachment of a lipid chain
• Modify an oligonucleotide and use it for DNA-directed immobilization
• Isolate an enzyme for in vitro synthesis of a polysaccharide
• Design a ligand for a protein by molecular modeling
• Perform a proteome analysis in yeast

All experiments haven been thoroughly tested in the authors' laboratory and come with an easy to follow step-by-step instruction, complemented by abundant practical hints and by a thorough treatment of the underlying theory and mechanisms.

Contents

1. Introduction: Chemical Biology - A New Science at the Crossroads of Chemistry and Biology
2. DNA-Synthesis and DNA-Hybridization
3. Doubly-Labeled Peptide Nucleic Acids as Probes for the Detection of DNA Point Mutations
4. Synthesis and Characterization of a Covalent Oligonucleotide-Streptavidin Conjugate for DNA-directed Immobilization
5. Solid Phase Synthesis of Peptides: Bradykinin Analogs and the Evaluation of Calcium Mobilization in PC-12 Cells
6. In silico Protein Ligand Design
7. Lipidation of Proteins and Peptides-Farnesylation of the Ras Protein
8. Insertion of Lipidated Peptides in Model Membranes
9. Isolation of Potato Phosphorylase and Enzymatic Synthesis of Amylose
10. Proteome Analysis-Identification of Proteins Isolated from Yeast
11. Lectins-Determination of the Sugar Specificity of Jacalin by a Sugar-Lectin Binding Assay (SLBA)
12. Combinatorial Synthesis and Genetic Algorithm
13. Solid-phase Synthesis of an Antibiotic

Appendix
Subject Index

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