Culture of Animal Cells provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells.

Culture of Animal Cells contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.

Culture of Animal Cells, Fifth Edition includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.

Contents:

1. Introduction
2. Biology of the Cultured Cell
3. Design and Layout of the Laboratory
4. Equipping the Laboratory
5. Aseptic Technique
6. Laboratory Safety and Biohazards
7. The Culture Environment: Substrate, Gas Phase, Medium and Temperature
8. Preparation and Sterilization
9. Disaggregation of the Tissue and Primary Culture
10. Maintenance of the Culture: Cell Lines
11. Cloning and Selection of Specific Cell Types
12. Physical Methods of Cell Separation
13. Characterization
14. Induction of Differentiation
15. The Transformed Phenotype
16. Contamination
17. Instability, Variation, and Preservation
18. Quantitation and Experimental Design
19. Measurement of Viability and Cytotoxicity
20. Culture of Specific Cell Types
21. Culture of Tumor Tissue
22. Three-Dimensional Culture Systems
23. Specialized Techniques

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ORDER NOW Culture of Animal Cells A Manual of Basic Technique Fifth Edition by R. Ian Freshney 2005 • 642 pages • $99.95 + shipping Texas residents please add 6.75 % sales tax