Directed Molecular Evolution of Proteins focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities.

It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity.

This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!

Contents

1. Introduction
2. Evolutionary Biotechnology -From Ideas and Concepts to Experiments and Computer Simulations
3. Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases
4. Construction of Environmental Libraries for Functional Screening of Enzyme Activity
5. Investigation of Phage Display for the Directed Evolution of Enzymes
6. Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process
7. Advanced Screening Strategies for Biocatalyst Discovery
8. Engineering Protein Evolution
9. Exploring the Diversity of Heme Enzymes through Directed Evolution
10. Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry
11. Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA
12. Evolutionary Generation versus Rational Design of Restriction Endonucleases with Novel Specificity
13. Evolutionary Generation of Enzymes with Novel Substrate Specificities

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