edited by Rita S. Dias
DNA Interactions with Polymers and Surfactants provides an extensive, up-to-date overview of the subject, giving readers a basis for understanding the factors leading to complexation between DNA and different cosolutes, including metal ions, polyelectrolytes, spermine, spermidine, surfactants and lipids, and proteins.
DNA Interactions with Polymers and Surfactants includes:
- Polyelectrolytes, physico-chemical aspects and biological significance
- Solution behavior of nucleic acids
- Single DNA molecules: compaction and decompaction
- Interaction of DNA with surfactants and cationic polymers
- Interactions of histones with DNA
- DNA-DNA interactions
- The hydration of DNA-amphiphile complexes
- DNA-surfactant/lipid complexes at liquid interfaces
- DNA and DNA-surfactant complexes at solid surfaces
- The role of correlation forces for DNA-cosolute interactions
- Simulations of polyions
- Cross-linked DNA gels and gel particles
- DNA as an amphiphilic polymer
- Lipid-DNA interactions
Contents
1. Polyelectrolytes. Physicochemical Aspects and Biological Significance
- Polyelectrolytes and Biological Function
- Electrostatic Interactions
- Solution Properties
- Flexibility
2. Solution Behavior of Nucleic Acids
- Biological Function of Nucleic Acids
- Discovery of DNA
- Structure of Nucleic Acids
- Nuclei Acids Nanostructures
- Behavior of DNA in Solution
- Melting of Double-Stranded DNA
3. Single DNA Molecules: Compaction and Decompaction
- Condensation and Compaction of DNA by Surfactants
- DNA Condensation by Cationic Liposomes
- DNA Compaction and Decompaction by Multivalent Cations
- DNA Compaction by Polycations
- Compaction of DNA in a Crowded Environment of Neutral Polymer
4. Interaction of DNA with Surfactants in Solution
- DNA–Cationic Surfactant Interactions
- DNA Covalent Gels and Their Interaction with Surfactants
5. Interaction of DNA with Cationic Polymers
- Theory of DNA Interacting with Polycations
- Condensation of DNA, Phase Diagram, and Structure
- Formation of Polycation–DNA Complexes: Polyplexes
- DNA-Nanoparticles for Gene Delivery
- Cellular Uptake and Intracellular Interactions of Polyplexes
6. Interactions of Histones with DNA: Nucleosome Assembly, Stability, Dynamics, and Higher Order Structure
- Histones
- Structure of Histone–DNA Complexes
- Assembly of Nucleosomes and Chromatosomes
- Stability and Dynamics of Nucleosomes
- Higher Order Chromatin Structures
7. Opening and Closing DNA: Theories on the Nucleosome
- Unwrapping Nucleosomes
- Nucleosome Sliding
- Transcription Through Nucleosomes
- Tail Bridging
8. DNA–DNA Interactions
- The Statistical Polymer Solution Model Predicts DNA Collapse/Aggregation Phase Behavior
- DNA in Solution is Condensed to a Compact State by Multivalent Cationic Ligands
- Ion Correlation Effects Included in Theory and in Computer Modeling Explain DNA–DNA Attraction
9. Hydration of DNA–Amphiphile Complexes
- General Properties of DNA Double Helices and Cationic Aggregates
- Thermodynamics of DNA–Amphiphile Complexes
- Molecular Properties of DNA–Amphiphile Complexes
10. DNA–Surfactant/Lipid Complexes at Liquid Interfaces
- Soluble Surfactants
- Insoluble Surfactant
- Lipids
- Mixtures of Surfactants and Lipids
11. DNA and DNA–Surfactant Complexes at Solid Surfaces
- Adsorption of DNA at Surfaces
- Attachment of DNA Surfaces—Strategies and Challenges
- DNA Structure on Surfaces—Comparison with Highly Charged Polyelectrolytes
- Some Applications—Arrays and Nanostamping
12. Role of Correlation Forces for DNA–Cosolute Interactions
- Experimental Evidence of DNA Condensation Induced by Electrostatic Agents
- Simulations Used to Characterize the DNA Compaction Mechanism
- Ion Correlations Limiting the Validity of DLVO Theory
- Ion Correlations Driving the Compaction of DNA
- Conformation of Compact DNA—The Coil to Toroid Transition
13. Simulations of Polyions: Compaction, Absorption onto Surfaces, and Confinement
- Models
- Solutions of Polyions with Multivalent Counterions
- Polyion Adsorption onto Charged Surfaces
- Polyions in Confined Geometries
14. Cross-linked DNA Gels and Gel Particles
- Covalently Cross-Linked DNA Gels
- ds-DNA versus ss-DNA: Skin Formation
- DNA Gel Particles
- Physical DNA Gels
15. DNA as an Amphiphilic Polymer
- Some General Aspects of Self-Assembly
- Illustrations
16. Lipid–DNA Interactions: Structure–Function Studies of Nanomaterials for Gene Delivery
- Formation and Structures of CL–DNA Complexes
- Effect of the Lipid–DNA Charge Ratio (rchg) on CL–DNA Complex Properties
- Effect of the Membrane Charge Density (sM) on CL–DNA Complex Properties
- Effect of Nonlamellar CL–DNA Complex Structure on the Transfection Mechanism
- Model of Transfection with Lamellar CL–DNA Complexes
- Model of Transfection with Inverted Hexagonal CL–DNA Complexes
- PEGylated CL–DNA Complexes: Surface Functionalization and Distinct DNA–DNA Interaction Regimes
Index
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