Enzyme Assays fills the need for a book presenting the most important methods for high-throughput screening and functional characterization of enzymes.
Contents
Part I: High-Throughput Screening
Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes
- Direct Assays Using Chromogenic Substrates
- Indirect Assays Using Coupled Reaction-pH Indicators
- Estimating and Measuring Selectivity
High-Throughput Screening Systems for Assaying the Enantioselectivity of Enzymes
- UV/Vis Spectroscopy-Based Assays
- Assays Using Fluorescence
- Assays Based on Mass Spectrometry
- Assays Based on Nuclear Magnetic Resonance Spectroscopy
- Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases
- Assays Based on Gas Chromatography
- Assays Based on HPLC
- Assays Based on Capillary Array Electrophoresis
- Assays Based on Circular Dichroism(CD)
- Assay Based on Surface-Enhanced Resonance Raman Scattering
High-Throughput Screening Methods Developed for Oxidoreductases
- High-Throughput Methods for Various Oxidoreductases
Industrial Perspectives on Assays
- Prerequisites for an Effective Biocatalyst Screening in Chemical Custom Manufacturing
- CCM Compliant Screening Methods Based on Optical Spectroscopy (UV/Vis and Fluorescence)
- CCM Compliant Screening Methods Based on Generic Instrumental Assays
Part II: Genetic Selection
Agar Plate-Based Assays
- Faciliated Screening-Based Methods
- In Vivo Selection-Based Methods
High-Throughput Screens and Selections of Enzyme-Coding Genes
- The Basics of High-Throughput Screens and Selections
- High-Throughput Selection of Enzymes Using Phage Display
- High-Throughput Selection of Enzymes Using Cell Display
- In Vivo Genetic Screens and Selections
- Screens for Heterologous Protein Expression and Stability
- In Vitro Compartmentalization
- IVC in Double Emulsions
Chemical Complementation
- Complementation Assays
- Development of Chemical Complementation
- Applications of Chemical Complementation
Molecular Approaches for the Screening of Novel Enzymes
- Use of Nucleic Acid Probes to Detect Enzyme-Coding Genes in Cultivated Microorganisms
- The Microbial Metagenome: A Resource of Novel Natural Products and Enzymes
Part III: Enzyme Fingerprinting
Fluorescent Probes for Lipolytic Enzymes
- Fluorogenic and Fluorescent Substrates for Enzyme Activity
- Fluorescent Inhibitors for Quantitative Analysis of Active Enzymes and Functional Enzyme Fingerprinting
Fingerprinting Methods for Hydrolases
- Hydrolase Fingerprinting
- Classification from Fingerprinting Data
Protease Substrate Profiling
- Functional Protease Profiling-Peptide Substrate Libraries
- Identification of Macromolecular Substrates
Enzyme Assays on Chips
- Immobilization Strategies
- Microarray-Based Methods for Detection of Enzymatic Activity
Index
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