This state-of-the-art reference provides a comprehensive review of enzyme function in human and animal health—covering basic principles and applications in antibiotic biosynthesis, biocatalysis, screening and assay optimization, as well as new and emerging technologies in the biotechnological and pharmaceutical industries.

Offers real-world examples of timely laboratory, pharmaceutical, and manufacturing processes—including large-scale applications of current enzyme manipulations!

Emphasizing modern methods of metabolic engineering and drug discovery processes, Enzyme Technologies for Pharmaceutical and Biotechnological Applications details:

• enzyme targets related to human disease
• the development of effective enzymatic assays
• up-to-the-minute methodologies in high-throughput screening
• the discovery of novel compounds for lead generation
• improvements in antibiotic yield
• the generation of contemporary protein products
• and much more!

Enzyme Technologies for Pharmaceutical and Biotechnological Applications is an authoritative and practical handbook for all enzymologists; organic, pharmaceutical, and medicinal chemists and biochemists; molecular and cell biologists; genetic, biochemical, and chemical engineers; microbiologists; and upper-level undergraduate and graduate students in these disciplines.

Contents

1. Biosynthesis
   1. d-(L-a-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase as a Model Tripeptide Synthetase
   2. Metabolic Engineering for Cephalosporin C Yield Improvement and Production of Intermediates
   3. Bioconversion of Penicillis to Cephalosporins
   4. Direct Fermentative Production of Acyltylosins by Genetically Engineered Strains of Streptomyces fradiae
   5. Engineering Streptomyces avermitilis for the Production of Novel Avermectins: Mutant Design and Titer Improvement

2. Biocatalysis
   6. Biocatalytic Syntheses of Chiral Intermediates for Antihypertensive Drugs
   7. Cloning, Structure, and Activity of Ketone Reductases from Baker’s Yeast
   8. Cross-Linked Enzyme Crystals: Biocatalysts for the Organic Chemist
   9. Enzymatic Deacylation of Echinocandins and Related Antifungal Agents

3. Screening/Optimization
   10. Roles of Enzymes in Antibacterial Drug Discovery
   11. Penicillin-Binding Proteins as Antimicrobial Targets: Expression, Purification, and Assay Technologies
   12. Development of a High-Throughput Screen for Streptococcus pneumoniae UDP-N-Acetylmuramoyl-Alanine: D-Glutamate Ligase (MurD) for the Identification of MurD Inhibitors
   13. Purification and Assay Development for Human Rhinovirus Proteases
   14. Screening for Parasiticides Using Recombinant Microorganisms
   15. Screening for Inhibitors of Lipid Metabolism
   16. Design and Development of a Selective Assay System for the Phospholipase A2 Superfamily

4. Emerging Technologies
   17. Understanding and Exploiting Bacterial Polyketide Synthases
   18. Polyketide Synthases: Analysis and Use in Synthesis
   19. Enzymatic Synthesis of Fungal N-Methylated Cyclopeptides and Depsipeptides
   20. New Strategies for Target Identification, Validation, and Use of Enzymes in High-Throughput Screening
   21. Use of Genomics for Enzyme-Based Drug Discovery
   22. Assigning Precise Function to Genes
   23. Redesigning Binding and Catalytic Specificities of Enzymes
   24. Proteomics: Chromatographic Fractionation Prior to Two-Dimensional Polyacrylamide Gel Electrophoresis for Enrichment of Low-Abundance Proteins to Facilitate Identification by Mass Spectrometric Methods

   Index

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   ORDER NOW Enzyme Technologies for Pharmaceutical and Biotechnological Applications edited by Herbert A. Kirst 611 pages • $198.95 + shipping Texas residents please add 6.75 % sales tax