From Genes to Genomes opens with a brief review of the basic concepts of molecular biology, before moving on to describe the key molecular methods and how they fit together.

From Genes to Genomes includes full two-colour design throughout and an accompanying website.

Specific changes for the new edition include:

• Strengthening of gene to genome theme
• Updating and reinforcing of material on proteomics, gene therapy and stem cells
• More eukaryotic/mammalian examples and less focus on bacteria

Contents

1. Basic molecular biology

• Nucleic acid structure
• What is a gene?
• Information flow: gene expression
• Gene structure and organization

2. How to Clone a Gene

• What is Cloning?
• Overview of the Procedures
• Gene Libraries
• Hybridization
• Polymerase Chain Reaction
• Extraction and Purification of Nucleic Acids
• Detection and Quantitation of Nucleic Acids
• Gel Electrophoresis

3. Cutting and Joining DNA

• Restriction Endonucleases
• Ligation
• Modification of Restriction Fragment Ends
• Other Ways of Joining DNA Molecules

4. Vectors

• Plasmid Vectors
• Vectors Based on the Lambda Bacteriophage
• Cosmids
• M13 vectors
• Expression Vectors
• Vectors for Cloning and Expression in Eukaryotic Cells
• Supervectors: YACs and BACs

5. Genomic and cDNA libraries

• Genomic Libraries
• Growing and Storing Libraries
• cDNA Libraries
• Random, Arrayed and Ordered Libraries

6. Finding the Right Clone

• Screening Libraries with Gene Probes
• Screening Expression Libraries with Antibodies
• Subcloning
• Characterization of Plasmid Clones

7. Polymerase Chain Reaction

• The PCR Reaction
• PCR in Practice
• Cloning PCR Products
• Long-Range PCR
• Reverse-Transcription PCR
• Rapid Amplification of cDNA Ends
• Quantitative PCR
• Applications of PCR

8. Characterization of a Cloned Gene

• DNA Sequencing
• Databank Entries and Annotation
• Sequence Analysis
• Sequence Comparisons
• Protein Structure
• Confirming Gene Function

9. Analysis of Gene Expression

• Analysing Transcription
• Methods for Studying the Promoter
• Regulatory Elements and DNA-Binding Proteins
• Translational Analysis

10. Products from Native and Manipulated Cloned Genes

• Factors Affecting Expression of Cloned Genes
• Expression of Cloned Genes in Bacteria
• Expression in Eukaryotic Host Cells
• Adding Tags and Signals
• In Vitro Mutagenesis
• Vaccines

11. Genomic Analysis

• Genome Sequencing
• Analysis and Annotation
• Comparing Genomes
• Genome Browsers
• Relating Genes and Functions: Genetic and Physical Maps
• Transposon Mutagenesis and Other Screening Techniques
• Conclusion

12. Analysis of Genetic Variation

• Single Nucleotide Polymorphisms
• Larger-Scale Variations
• Other Methods for Studying Variation
• Human Genetic Diseases
• Molecular Phylogeny

13. Post-genomic Analysis

• Analysing Transcription; Transcriptomes
• Array-Based Methods
• Translational Analysis; Proteomics 3
• Post-Translational Analysis: Protein Interactions
• Integrative Studies; Systems Biology

14. Modifying Organisms; Transgenics

• Modification of Bacteria and Viruses: Live Vaccines
• Transgenesis and Cloning
• Animal Transgenesis
• Applications of Transgenic Animals
• Transgenic Plants and Their Applications

Index

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