Fundamentals of Contemporary Spectrometry gives readers a concise and authoritative overview of modern mass spectrometry instrumentation, techniques, and applications, including the latest developments.
Fundamentals of Contemporary Mass Spectrometry gives scientists in diverse disciplines a practical foundation in modern mass spectrometry.
Contents
Instrumentation
1. Basics of Mass Spectrometry
- Brief History of Mass Spectrometry
- Desirable Features of Mass Spectrometry
- Basic Principles of Mass Spectrometry
- Anatomy of a Mass Specturm
2. Modes of Ionization
- Why Ionization in Required
- General Construction of an Ion Source
- Electron Ionization
- Chemical Ionization
3. Mass Analysis and Ion Detection
- Mass Resolving Power
- Kinetic Energy of Ions
- Magnetic-Sector Mass Spectrometers
- Quadrupole Mass Spectrometers
4. Tandem Mass Spectrometry
- Basic Principles of Tandem Mass Spectrometry
- Types of Scan Functions
- Ion Activation and Dissociation
- Reactions in Tandem Mass Spectrometry
5. Hyphenated Separation Techniques
- Benefits of Coupling Separation Devices with Mass Spectrometry
- General Considerations
- Chromatographic Properties
- Gas Chromatography/Mass Spectrometry
Organic and Inorganic Mass Spectrometry
6. Organic Mass Spectrometry
- Determination of Molecular Mass
- Molecular Formula from Accurate Mass Values
- Molecular Formula from Isotopic Peaks
- General Guidelines for Interpretation of a Mass Spectrum
7. Inorganic Mass Spectrometry
- Ionization of Inorganic Compounds
- Thermal Ionization Mass Spectrometry
- Spark-Source Mass Spectrometry
- Glow Discharge Ionization Mass Spectrometry
8. Proteins and Peptides: Structure Determination
- Structure of Proteins
- Determination of the Sequence of a Protein
- General Protocol for Amino Acid Sequence Determination of Proteins
- Molecular Mass Measurement of Proteins
9. Proteins and Peptides: Posttranslational Modifications
- Traditional Approaches to Identify Disulfide Bonds
- Mass Spectrometry-Based Methods to Identify Disulfide Bonds
- Labeling for the Analysis of Phosphoproteins
- Mass Spectrometry Protocol for the Analysis of Phosphoproteins
10. Proteins and Peptides: Higher-Order Structures
- Charge-State Distribution
- Hydrogen-Deuterium Exchange to Study Conformational Stated of Proteins
- Chemical Cross-Linking as a Probe for the Three-Dimensional Structure of Proteins
- Ion Mobility Measurements to Study Protein Conformational Changes
11. Structural Diversity in Oligosaccharides
- Classes of Glycans
- Mass Spectrometric Methods for Complete Structure Elucidation of Oligosaccharides
12. Characterization of Lipids
- Classification and Structures of Lipids
- Mass Spectrometry of Fatty Acids and Acylglycerols
- Mass Spectrometry of Phospholipids
- Mass Spectrometry of Glycolipids
13. Structure Determination of Oligonucleotides
- Structures of Nucleotides and Oligonucleotides
- Mass Spectrometry Analysis of Nucleosides and Nucleotides
- Cleavage of Oligonucleotides
- Molecular Mass Determination of Oligonucleotides
14. Quantitative Analysis
- Advantages of Mass Spectrometry
- Data Acquisition
- Calibration
- Validation of a Quantitative Method
15. Miscellaneous Topics
- Enzyme Kinetics
- Imaging Mass Spectrometry
- Analysis of Microorganisms
- Clinical Mass Spectrometry
Index
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