Features:

• Covers state-of-the-art technologies, including the latest information on gene manipulation methods
• Includes a broad range of practical applications
• Explains key principles and concepts behind the methods covered
• Contains clearly detailed, step-by-step explanations of protocols with helpful troubleshooting tips

Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods.

The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects.

Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies

This unique resource provides the tools for success in molecular and cellular biology research today.

Contents

1. Approaches to Novel Research.
2. Rapid Isolation of Specific cDNAs or Genes by PCR
3. High Throughput mRNA Profiling via cDNA Microarrays or DNA Chips
4. mRNA Profiling by Quantitative RT-PCR Technique
5. Functional Genome vs. Drug Discovery
6. Construction and Screening of Subtracted and Complete Expression cDNA Libraries
7. Subcloning of Gene or cDNAs
8. Nonisotopic and Isotopic DNA or RNA Sequencing
9. Information Superhighway and Computer Databases of Nucleic Acids and Proteins
10. Characterization of DNA or Genes by Southern Blot Hybridization
11. Gene Overexpression by Sense RNA in Mammalian Systems
12. Analysis of Gene Expression at the RNA Level
13. Analysis of Gene Expression at the Protein Level
14. Proteomics vs. Drug Discovery
15. Phage Display Technology
16. DNA or mRNA Analysis by Radioactive in situ Hybridization RT-PCR Detection of Low Abundance mRNAs
17. Isolation and Characterization of Genes from Genomic DNA Libraries
18. Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression Studies
19. New Strategies for Gene Knockout
20. Generation of Random Chimeras for Functional Assays of Genes
21. Rapid Generation of Mutant DNA Constructs by Site-Specific Mutagenesis
22. Large-scale Expression and Purification of Recombinant Proteins

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