Gene Silencing by RNA Interference:

• Presents the latest information in this new and rapidly evolving field
• Shares tried and tested methods and step-by-step protocols written by experts in the field
• Covers the most interesting model cell systems, from cultured cells to animals and plants
• Provides a "one-stop" practical guide to gene silencing by RNA interference
• Combines essential background to the RNAi field with practical techniques

Contents

• Gene silencing by RNA interference and the role of small interfering RNAs
• Basics of siRNA design and chemical synthesis
• Oligonucleotide scanning arrays in the design of small interfering RNAs
• siRNA production by in vitro transcription
• Production of siRNAs with the application of deoxyribozymes
• Production of siRNA in vitro by enzymatic digestion of double-stranded RNA
• Plasmid-mediated intracellular expression of siRNAs
• Lentiviral vector-mediated delivery of si/shRNA
• Exogenous siRNA delivery: protocols for optimizing delivery to cells
• RNAi in Drosophila cell cultures
• RNAi in Caenorhabditis elegans
• Delivery of RNAi reagents in C. elegans by microinjection
• RNAi in Drosophila embryos
• RNAi in Xenopus laevis oocytes
• RNAi in Xenopus embryos
• An RNAi-based genomic library for forward genetics in the African Trypanosome
• RNAi in the malaria parasite Plasmodium
• RNA in transgenic animal models
• RNA interference in mouse model
• Virus-induced RNA silencing in plants
• RNA silencing in plants - biolistic delivery of RNAi reagents
• Agro-infiltration: a versatile tool for RNAi studies in plants

Index

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