Second Edition
by Gennady P. Manchenko

• Provides the most thorough collection of step-by-step zymogram procedures available
• Clearly explains the principles of in-gel visualization of enzymes after electrophoretic separation
• Provides diagrams of biochemical reaction sequences for each detection method, thereby enabling the most correct interpretation of the resultant zymograms
• Enables readers to select the most appropriate zymogram technique according to the question to be answered and the laboratory equipment and resources available
• Presents enzymes in numerical order according to their EC numbers and most recent recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology

Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date.

New in the Second Edition:

• An overview of new applications of enzyme electrophoresis and zymogram techniques
• Zymogram techniques for approximately 100 enzymes not included in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes
• Information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms
• An appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels

The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology.

Contents

1. Introduction
   1. Enzyme Electrophoresis
   2. Zymogram Techniques
   3. Genetic Bases of Multiple Molecular Forms of Enzymes Developed on Zymograms Applications

2. General Principles of Enzyme Detection on Electrophoretic Gels
   4. Chromogenic Reactions
   5. Fluorogenic Reactions
   6. Autoradiography
   7. Bioautography
   8. Two-Dimensional Gel Spectroscopy
   9. Immunoblotting

3. Methods of Detection of Specific Enzymes
   10. The Structure of Enzyme Sheets
   11. General Considerations, Comments, and Recommendations
   12. Enzyme Sheets

Appendices

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