High Performance Capillary Electrophoresis
Theory, Techniques, and Applications
edited by Morteza G. Khaledi

This book discusses specific topics which are not treated extensively by other books including two-dimensional separations, CE on microchips, nonaqueous CE, indirect detection, monitoring enzymatic reactions, and more.

High Performance Capillary Electrophoresis contains chapters which cover:

• Theory and Principles of the Six HPCE Techniques
  
• Detection Systems, Including Indirect Detection
  
• Essential Operation Topics Such as Sample Introduction and Stacking, Coated Capillaries, and Method Validation
  
• Recently Developed Methods, Including Two-Dimensional Separations, Nonaqueous CE, and HPCE on Microchips
  
• All of the Basic HPCE Applications, With an Emphasis on Bioanalytical Uses
  
• HPCE in the Determination of Physico-Chemical Properties of Molecules
  
• And More!

Contents

Part I: Theory and Modes of HPCE

1. Capillary Electrophoresis: Overview and Perspective
   
2. Theory of Capillary Zone Electrophoresis
   
3. Micellar Electrokinetic Chromatography
   
4. Band Broadening in Micellar Electrokinetic Chromatography
   
5. Capillary Gel Electrophoresis
   
6. Capillary Isoelectric Focusing
   
7. Capillary Isotachophoresis
   
8. Capillary Electrochromatography

   Part II: Detection Systems in HPCE

9. Capillary Electrophoretic Detectors Based on Light
   
10. Electrochemical Detection in High-Performance Capillary Electrophoresis
    
11. Indirect Detection in Capillary Electrophoresis
    
12. High-Performance Capillary Electrophoresis-Mass Spectrometry

    Part III: Operational Aspects and Special Techniques in HPCE

13. Sample Introduction and Stacking
    
14. Coated Capillaries in High-Performance Capillary Electrophoresis
    
15. Nonaqueous Capillary Electrophoresis
    
16. Method Validation in Capillary Electrophoresis
    
17. Two-Dimensional Separations in High-Performance Capillary Electrophoresis
    
18. Microfabricated Chemical Separation Devices

    Part IV: Applications of HPCE

19. Peptides Analysis by Capillary Electrophoresis: Methods Development and Optimization, Sensitivity Enhancement Strategies, and Applications
    
20. Capillary Electrophoresis of Proteins
    
21. Capillary Electrophoresis of Carbohydrates
    
22. DNA Sequencing by Multiplexed Capillary Electrophoresis
    
23. Chiral Separations by Capillary Electrophoresis
    
24. Capillary Electrophoresis of Inorganic Ions
    
25. The Analysis of Pharmaceuticals by Capillary Electrophoresis
    
26. On-Line Immunoaffinity Capillary Electrophoresis for the Determination of Analytes Derived From Biological Fluids
    
27. Microbioanalysis Using On-Line Microreactors-Capillary Electrophoresis Systems
    
28. Electrophoretically Mediated Microanalysis

    Part V: Physicochemical Studies

29. Affinity Capillary Electrophoresis: Using Capillary Electrophoresis to Study The Interactions of Proteins with Ligands
    
30. Determination of Physicochemical Parameters by Capillary Electrophoresis
    
31. Applications of Micellar Electrokinetic Chromatography in Quantitative Structure-Activity Relationship Studies: Estimation of Log P_ow and Bioactivity

Appendices
Index

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