Identification of Microorganisms by Mass Spectrometry is a multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis.

Features:

• Metastable atom bombardment pyrolysis mass spectrometry
• Matrix-assisted laser desorption/ionization mass spectrometry (MALDI)
• MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria
• High-resolution Fourier transform mass spectrometry (FTMS)
• Electrospray ionization (ESI) mass spectrometry

Contents

Cultural, Serological and Genetic Methods for Identification of Bacteria

• Identification of bacteria by cultural methods
• Identification of bacteria by serological methods
• Identification of bacteria by genetic methods
• Other methods used for bacterial characterization

Mass Spectrometry: Identification and Biodetection, Lessons Learned and Future Developments

• Analysis of Fatty Acid and Sugar Monomers using GC-FID, GC-MS and GC-MS-MS
• Analysis of PCR products using PCR, PCR-MS and PCR-MS-MS
• Analysis of proteins using MALDI-TOF MS
• Chemical markers for protein-based identification or biodetection

An Introduction to Maldi TOF MS Analysis of Whole Bacteria

• Mass Spectrometry and Time-of-Flight MS
• Matrix Assisted Laser Desorption Ionization
• MALDI/TOF Mass Spectrometry
• MALDI TOF and Bacterial Identification

The Development of the Block II Chemical Biological Mass Spectrometer

• Development History and Design Philosophy
• Requirements and Specifications
• Performance Testing

Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry

• Sample Preparation for Rapid, Reproducible Cell Culture
• Analytical instrumentation for sensitive detection and spectral reproducibility
• Spectral library assembly
• Pattern Recognition Methods for Objectively Classifying Bacteria

MALDI TOF Mass Spectrometry of Intact Bacteria

• MALDI MS of Cellular Extracts
• Taxonomy: From Isolates to Whole Cell MALDI
• Whole Cell MALDI MS
• Biology Based Changes in Whole Cell MALDI Spectra
• Analysis of Mixtures
• Experimental Approaches
• Identification of Protein Markers
• Analysis of Target Proteins
• Analysis of Clinical Isolates

Development of Spectral Pattern Matching Approaches to Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Bacterial Identification

• MALDI-MS Signature Library Construction and Identification

Studies of Malaria by Mass Spectrometry

• Plasmodium in Red Blood Cells
• Experimental Protocols for LDMS Detection of Malaria
• Malaria Detection by Laser Desorption Mass Spectrometry
• MS-Based Proteomics of the Plasmodium Parasite

Bacterial Strain Differentiation by Mass Spectrometry

• Analysis of Cellular Proteins by Mass Spectrometry
• Application of MALDI-TOF to Bacteria Identification

Bacterial Protein Biomarker Discovery: A Focused Approach to Developing Molecular Based Identification Systems

• Protein Extraction Methods
• Mass Spectrometry
• Automating the Process
• Collecting and Sequencing Proteins

High Throughput Microbial Characterisations Using Electrospray Ionisation Mass Spectrometry and Its Role in Functional Genomics

• Microbial characterisations beyond the genomic level: Functional genomics
• Electrospray (ionization) mass spectrometry (ESMS)
• ESMS of Microbes
• Direct infusion ESMS of crude cell extracts for high-throughput characterizations - metabolic fingerprinting and footprinting

Bioinformatics for Flexibility, Reliability and Mixture Analysis of Intact Microorganisms

• Library Matching
• Machine Learning
• Bioinformatics
• Protein Molecular Masses
• Protein Maps
• Microsequences from Peptides and Proteins

MALDI-FTMS of Whole Cell Bacteria

• Fundamentals of MALDI-FTMS
• Fundmentals of Complex Biological Analysis
• Whole Cell Characterization Through MALDI-FTMS
• Recombinant Over-Expressed Proteins Desorbed Directly from Whole Cells

A Review of Antibody Capture and Bacteriophage Amplification in Connection with the Direct Analysis of Whole Cell Bacteria by MALDI-TOF-MS

• Bacterial Identification
• Immunocapture of Bacterial Mixtures
• Bacteriophage Amplification of Bacteria

Discrimination and Identification of Microorganisms by Ppyrolysis Mass Spectrometry: From Burning Ambitions to Cooling Embers - A Historical Perspective

• Introduction to microbial characterisation
• Principles of PyMS
• Early developments and investigations (1952 to 1985)
• The mid 1980s and beyond
• The move from cluster analyses to neural networks
• Reproducibility of PyMS

Index

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