Industrial Bioseparations offers a careful balance between the fundamentals of bioseparations processing and the practical applications in industry today.

Industrial Bioseparations is laid out in a methodical way with preliminary chapters covering general approaches to bioseparations for commercially important biomacromolecules, thermodynamics and mass transfer principles, and following chapters addressing unit operations such as filtration and chromatography.

Contents

1. Introduction to Bioseparations

• Statement of the problem
• Engineering Developments
• Current approaches to bioseparations

2. Thermodynamics

• A brief introduction
• Thermodynamics of open systems
• Thermodynamic analysis of processes

3. Principles of Mass Transfer

• Diffusion
• Fluxes
• Law of conservation of mass

4. General Properties of Bioproducts

• Proteins
• Nucleic Acids
• Laboratory experiments

5. Cell disruption

• General approach to cell disruption
• Bead Mills
• Homogenizers
• Chemical Methods
• Biochemical Methods
• Laboratory experiments

6. Separation of Solids

• General Principles
• Filtration
• Centrifugation
• Sedimentation
• Laboratory experiments
• Liquid/liquid extraction

7. Chromatography

• General Principles
• Gel permeation
• Ion Exchange Chromatography
• Hydrophobic Interactions Chromatography
• Affinity Chromatography
• Simulated Moving Bed Chromatography
• Laboratory Experiments

8. Protein Precipitation

• Precipitation by salting-out
• Isoelectric Precipitation
• Precipitation by non-ionic polymers
• Laboratory Experiments

9. Polishing

• Freeze drying
• Crystallization
• Product Formulation
• Laboratory Experiments

10. Separations in a Chip

• Introduction to micro-devices
• Fluidics
• Micro-reactors, micro-mixers, and valves
• Chromatography and electrophoresis in a chip
• Micro-sensors

11. Design of Separation cascades

• General Principles
• Examples
• Laboratory Projects

Index

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