A Discovery-Based Laboratory Course
by Guy A. Caldwell
Integrated Genomics introduces the excitement of discovery to the basic molecular biology laboratory.
Features:
- Provides training with multiple inexpensive and popular model organisms including C.elegans and S.cerevisiae
- Adopts a student-tested, discovery-based format
- Integrates basic bioinformatics within an experimental context
- Inclusive of modern molecular genomic/proteomic technologies such as the yeast two-hybrid system and RNAi
- Accompanying website includes easy access to instructional materials and experimental reagents including all strains and plasmids used in the course
Contents
- Introduction to Basic Laboratory Genetics
- Gene Expression Analysis Using Transgenic Animals
- Creation and Testing of Transgenic Yeast For Use In Protein-Protein Interaction Screening
- Yeast Two-Hybrid Screening
- Isolation and Identification of Interacting Proteins
- Using Bioinformatics in Modern Science
- Generation of an RNAi Vector
- RNA-Mediated Interference by Bacterial Feeding
Appendices
- Recombinational Cloning
- Recipes and Media Preparation
- Steriale Techniques and Worm Protocols
- Mutant C. elegans Phenotypes
- Vectomr Maps
Index
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