edited by Rajni Hatti-Kaul
Isolation and Purification of Proteins details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, and product formulation, and regulatory and commercial considerations in protein production.
Contents
- Resolution technique for protein separation
- Selectivity in affinity chromatography
- Genetic approaches to facilitate purification
- Solid-liquid separation
- Membranes for protein isolation and purification
- Precipitation of proteins - non-specific and specific
- Protein crystallization for large-scale bioseparation
- Extraction for rapid protein isolation
- Absorption as an initial step for the capture of proteins
- Fast chromatography of proteins
- Novel approaches to the chromatography of proteins
- Scale up and commercial production of recombinant proteins
- Process monitoring in downstream processing
- Protein formulation
- Regulatory requirements
- Cleaning and sanitization issues in protein purification
Index
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