Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.

Features:

• Describes basic laboratory techniques in detail, accompanied by practical examples
• Contains an outstanding selection of references for follow-up work
• Includes cross-referencing of methods allowing for easy access to information
• Illustrates the importance of ethical considerations and safety in laboratory research
• Features introductory material for each chapter, providing a better understanding of the methods and applications
• Examines potential problems and notes trouble-shooting tips

Making and Using Antibodies: A Practical Handbook covers:

• Commercially available adjuvants designed for the production of antisera in the research setting
• Methods for the production, purification, and characterization of antibodies
• Practical guidance to researchers needing to modify antibodies
• Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry
• Methods for applying immunolabeling to the realm of electron microscopy (EM)
• New antibody sources and new ways to use antobodies

Contents

1. Antibodies
2. Antigens
3. Adjuvants
4. Production of Polyclonal Antibodies
5. Production of Monoclonal Antibodies
6. Quantitative Production of Monoclonal Antibodies
7. Purification and Characterization of Antibodies
8. Making Antibodies in Bacteria
9. Chemical and Proteolytic Modification of Antibodies
10. Applications
11. Immunohistochemical Methods
12. Immunoelectron Microscopy
13. Flow Cytometry
14. ELISAs
15. Antibodies in the Future: Challenges and Opportunities

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