MALDI MS gives an in-depth description of the many different applications of MALDI MS, along with a detailed discussion of the technology itself.
Contents
The MALDI Process and Method
- Analyte Incorporation
- Absorption of the Laser Radiation
- The Ablation/ Desorption Process
- Ionization
- Fragmentation of MALDI Ions
- MALDI of Non-Covalent Complexes
- The Correct Choice of Matrix: Sample Preparation
MALDI Mass Spectrometry Instrumentation
- Lasers for MALDI-MS
- Fragmentation of MALDI Ions
- Mass Analyzers
- Fourier Transform Ion Cyclotron Resonance Mass Spectrometers
- Quadrupole Ion Trap Mass Spectrometers
- Hybrid Mass Spectrometers
- Future Directions
MALDI-MS in Protein Chemistry and Proteomics
- Sample Preparation for Protien and Peptide Analysis by MALDI MS
- Strategies for Using MALDI-MSin Protein Biochemistry
- Applications of MALDI-MS in Proteomics
- Computational Tools for Protein Analysis by MALDI-MS
- Clinical Applications of MALDI-MS
Microprobing and Imaging MALDI for Biomarker Detection
- History of Mass Spectrometry Imaging and Microprobing Techniques
- Visualization of Mass Spectrometric Information
- MALDI in Micro Dimensions: Instruments and Mechanistic Differences
- The Matrix Deposition Problem in High-Resolution Imaging
- Organisms, Organs, Tissues and Cells: Imaging MALDI at Various Lateral Resolutions
- Whole-Cell and Single-Cell Analysis: Prospects and Limitations
- Cell Sorting and Capturing
- Future Areas of Application
- Identification and Characterization: Limitations Due to Mas Resolution and Accuracy
MALDI-MS of Nucleic Acids and Practical Implementations in Genomics and Genetics
- Challenges in Nucleic Acid Analysis by MALDI-MS
- Genetic Markers
- Assay Formats for Nucleic Acid Analysis by MALDI-MS
- Applications in Genotyping
- Applications of Comparative Sequence Analysis
- Applications in Quantitation of Nucleic Acids for Analysis of Gene Expression and Gene Amplification
MALDI-MS of Glycans
- N-Glycosylation.
- O-Glycosylation
MALDI-MS of Lipids
- Analysis of Individual Lipid Classes and their Characteristics
- MALDI-TOF MS of Typical Lipid Mixtures
- Quantitative Aspects of MALDI-TOF MS of Lipids
- Problems, and their Potential Solution
MALDI-MS for Polymer Characterization
- Technical Aspects of MALDI-MS
- Attributes and Limitations of MALDI-MS
Small-Molecule Desorption/Ionization Mass Analysis
- Matrix Choices for Small-Molecule MALDI
- Sample Preparation
- Qualitative Characterization of LMM Molecules
- Analyte Quantitation by MALDI
- Separation Methods Coupled with MALDI and DIOS
Index
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