Focusing on the applications of all current microscopic techniques applied in biotechnology, this book presents the various methods as well as their suitability and limitations for a specific question. One especially valuable feature is the presentation of useful applications providing all basic information on the structure of the relevant objects. Readers are thus able to choose the most suitable applications for any specific problem.

In-depth background information on structure-function relationships is given and sample preparation with respect to a particular technique and explain the necessary equipment is explained.

Microscopic Techniques in Biotechnology is an essential reference for all those concerned with biotechnology.

Contents

1. Light Microscopy
   
   • Applications and Limitations in Light Microscopy
   • Useful Applications
   • Applications and Limitations of Confocal Laser Scanning Microscopes
   • Instrumentation and Preparation Techniques

2. Electron Microscopy
   
   • Applications and Limitations in Transmission Electron Microscopy
   • Imaging of Cellular and Large Subcellular Structures
   • Applications and Limitations in Scanning Electron Microscopy
   • Preparation Techniques

3. Other Microscopial Methods and Their Application Potential
   
   • Total Internal Reflection Microscopy
   • Multiphoton Excitation Microscopy
   • Analytical Features of Fluorescence Microscopes
   • Fluorescence Correlation Spectroscopy
   • Soft X-ray Microscopy
   • Microtomographic Techniques
   • Microscopic Imaging with Protons and Heavy Ions
   • Ultrasound Biomicroscopy and Scanning Acoustic Microscopy
   • Optical Coherence Microscopy/Tomography
   • Scanning Probe Microscopy
   • Near-Field Scanning Optical Microscopy

Index

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