This new edition of Natural Products Isolation has been significantly updated to reflect recent advances in the field, and has also been expanded with three new chapters on:

• hyphenated techniques
• purification by solvent extraction using partition coefficient
• isolation of microbial natural products

The book includes powerful cutting-edge techniques and simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies.

The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents

1. Natural Product Isolation
2. Initial and Bulk Extraction
3. Supercritical Fluid Extraction
4. An Introduction to Planar Chromatography
5. Isolation of Natural Products by Low-Pressure Column
6. Isolation by Ion-Exchange Methods
7. Separation by High-Speed Countercurrent Chromatography
8. Isolation by Preparative High-Performance Liquid Chromatography
9. Hyphenated Techniques
10. Purification by Solvent Extraction Using Partition Coefficient
11. Crystallization in Final Stages of Purification
12. Dereplication and Partial Identification of Compounds
13. Extraction of Plant Secondary Metabolites
14. Isolation of Marine Natural Products
15. Isolation of Microbial Natural Products
16. Purification of Water-Soluble Natural Products
17. Scale-Up of Natural Product Isolation
18. Follow-Up of Natural Product Isolation

Index

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