Features:

• Includes classic and current protein techniques

• Describes the principles and reactions for most protein techniques of extraction, purification, and characterization

• Presents useful advice on which techniques to use and when to apply a particular method

Principles and Reactions of Protein Extraction, Purification, and Characterization describes the principles and reactions for most of the techniques of protein extraction, purification, and characterization. The author focuses on the principles and mechanisms of the experimental procedures and describes the reactions involved. He includes useful advice on which techniques to use and when to apply a particular method.

The book discusses the advantages and disadvantages of the various protein techniques, enabling students and researchers to master the mechanisms behind the protocols. Seven chapters cover most protein techniques in a consistent format that makes the material easy to retrieve.

Contents

1. Extraction of Proteins
2. Estimation of Proteins
3. Electrophoretic Analyses of Proteins
4. Putrifaction of Proteins
5. Purification of Glycoproteins and Analyses of their Oligosaccharides
6. Antibodies: Structures, Interactions and Production
7. Antibody Labeling, Antibody Detection and Immunochemical Techniques

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