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Protein Science Reference book from C.H.I.P.S.

Protein Purification Techniques
Second Edition
Edited by Simon Roe

Protein Purification Techniques provides detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. It covers the major laboratory techniques and common sources of protein.

Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations.

Contents

  1. Purification strategy
  2. Getting started
  3. Analysis of purity
  4. Clarification techniques
  5. Cell disintegration and extraction techniques
  6. Concentration of the extract
  7. Separation on the basis of chemistry
  8. Chromatography on the basis of size
  9. Purification by exploitation of activity
  10. Scale up considerations
Appendix I: List of suppliers

Index

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Protein Purification Techniques
Second Edition
Edited by Simon Roe

262 pages • $191.00 + shipping
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