Protein Sequencing and Identification Using Tandem Mass Spectrometry
by Michael Kinter and Nicholas E. Sherman
This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.
Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing.
Protein Sequencing and Identification Using Tandem Mass Spectrometry includes:
- Peptide fragmentation and interpretation of product ion spectra
- Basic polyacrylamide gel electrophoresis
- Preparation of protein digests for sequencing experiments
- Mass spectrometric analysis using capillary liquid chromatography
- Techniques for protein identification by database searches
- Characterization of modified peptides using tandem mass spectrometry
- and much more!
Contents:
- An Introduction to Protein Sequencing Using Tandem Mass Spectrometry.
- The Primary Structure of Proteins and a Historical Overview of Protein Sequencing.
- Fundamental Mass Spectrometry.
- Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra.
- Basic Polyacrylamide Gel Electrophoresis.
- The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments.
- Mass Spectrometric Analysis of Tryptic Digests.
- Protein Identification by Database Searching.
- Sequence Analysis of Novel Proteins.
- The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry
Index
to see books • videos • cd-roms of related interest