Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research.

Features:

• Contains a compilation of modern applied proteomics methods which cover the entire process, from sample preparation to identification of potential drug targets and biomarkers of disease.
• Demonstrates strategies for protein recovery from samples that are resistant to conventional solubilization methods
• Provides practical examples of sample pre-fractionation for analysis of low abundance proteins
• Provides a good summary of recent improvements in the field of Two-Dimensional Gel Electrophoresis (2DGE)
• Describes optimized strategies for protein visualization in 2-D gels
• Encompasses automated liquid phase separation methods employing high-performance liquid chromatography (HPLC)
• Illustrates the use of Nuclear Magnetic Resonance (NMR) for the analysis of protein-DNA, protein-lipid, and protein-carbohydrate interactions

Contents

Sample Preparation

• Applications of Pressure Cycling Technology (PCT) in 2DGE
• Applications of Ion-Exchange Chromatography (IEX) to Reduce Sample Complexity Prior to Two-Dimensional Gel Electrophoresis (2DGE)
• Use of Camelid Antibody Fragments in the Depletion and Enrichment of Human Plasma Proteins for Proteomics Applications
• High-Throughput Plasma Depletion with Chicken Antibodies for Proteomic Analysis
• Immunoaffi nity Depletion of High-Abundant Proteins for Proteomic Sample Preparation
• Isolation of Plasma Membrane Proteins for Proteomic Analysis
• New Ultrafiltration and Solid Phase Extraction Techniques Improve Serum Peptide Detection

Sample Prefractionation and Analyses

• Tools for Sample Preparation and Prefractionation in Two-Dimensional Gel Electrophoresis
• Optic Nerve Fractionation for Proteomics
• Fractionation of Retina for Proteomic Analyses
• Reducing Protein Sample Complexity with Free Flow Electrophoresis (FFE)

Applications of Electrophoresis in Proteomics

• Destreaking Strategies for Two-Dimensional Electrophoresis
• Proteomic Approaches to the Study of Rheumatoid Arthritis
• Immunoglobulin Patterns in Health and Disease
• Difference Gel Electrophoresis (DIGE)
• Principles and Challenges of Basic Protein Separation by Two-Dimensional Electrophoresis
• Multidimensional Separation of Membrane Proteins
• Structural Approaches to Glycoproteomics
• Enrichment and Analysis of Glycoproteins in the Proteome

Applications of High-Performance Liquid Chromatography

• Proteomic Analyses Using High-Efficiency Separations and Accurate Mass Measurements
• Middle-Out Proteomics: Incorporating Multidimensional Protein Fractionation and Intact Protein Mass Analysis as Elements of a Proteomic Analysis Workflow
• Polymeric Monolithic Capillary Columns in Proteomics

Related Techniques

• Staining Proteins in Polyacrylamide Gels
• Multiplexed Proteomics: Fluorescent Detection of Proteins, Glycoproteins, and Phospoproteins in Two-Dimensional (2D) Gels
• Direct Immunoprobing on Glyoxyl Agarose Composite Gels
• Nuclear Magnetic Resonance-Driven Chemical Proteomics: The Functional and Mechanistic Complement to Proteomics
• Electrophoretic Nuclear Magnetic Resonance: Toward High-Throughput Structural Characterization of Biological Signaling Processes

Index

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