edited by John E. Coligan,
Ben M. Dunn, David W. Speicher
and Paul T. Wingfield

Short Protocols in Protein Science provides condensed descriptions of more than 500 protocols compiled from Current Protocols in Protein Science. Drawing from both the original "core" manual as well as the quarterly update service, this compendium includes all step-by-step descriptions of the principal methods covered in Current Protocols in Protein Science.

Contents

1. Strategies of Protein Purification and Characterization
2. Computational Analysis
3. Detection and Assay Methods
4. Extraction, Stabilization, and Concentration
5. Production of Recombinant Proteins
6. Purification of Recombinant Proteins
7. Characterization of Recombinant Proteins
8. Conventional Chromatographic Separations
9. Affinity Purification
10. Electrophoresis
11. Chemical Analysis
12. Post-Translational Modification: Glycosylation
13. Post-Translational Modification: Phosphorylation and Phosphatases
14. Post-Translational Modification: Specialized Applications
15. Chemical Modification of Proteins
16. Mass Spectrometry
17. Preparation and Handling of Peptides
18. Identification of Protein Interactions
19. Quantitation of Protein Interactions
20. Peptidases
21. Gel-Based Proteome Analysis

Appendix 1: Reagents and Solutions
Appendix 2: Useful Measurements and Data
Appendix 3: Commonly Used Techinques
Appendix 4: Selected Suppliers of Reagents and Equipment

Index

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