Structural Genomics on Membrane Proteins provides an excellent overview on novel research in bioinformatics and modeling on membranes, as well as the latest technological developments being employed in expression, purification, and crystallography to obtain high-resolution structures on membrane proteins.

Features:

• Discusses the various methods for production of recombinant membrane proteins in bacteria, yeast, insect, and mammalian cells
• Covers downstream processing, including solubilization, refolding, and purification
• Provides examples of studies on whole genomes of specific organisms, types of genes/proteins, and gene protein families
• Includes sections on the supply of protein for purification and crystallization attempts
• Presents alternative methods including the use of nuclear magnetic resonance and electron microscopy

Contents

• Bioinformatics in Membrane Protein Analysis
• Prokaryotic Membrane Transport Proteins: Amplified Expression and Purification
• Membrane Protein Production Strategies for Structural Genomics
• Refolding of Membrane Proteins for Large-Scale Production
• Crystallization of Membrane Proteins
• Signaling through Membrane Proteins
• Expression of Membrane Proteins in Yeasts
• Expression of Functional Membrane Proteins in the Baculovirus-Insect Cell System: Challenges and Developments
• Expression of Membrane Proteins in Mammalian Cells
• Solubilization and Purification of Membrane Proteins
• Fluorescent Labelling of Membrane Proteins in Living Cells
• Membrane Protein NMR
• Miniaturization of Structural Biology Technologies - From Expression to Biophysical Analyses
• Electron Microscopy and Atomic Force Microscopy of Reconstituted Membrane Proteins
• Structural Genomics Networks for Membrane Proteins
• Molecular Modelling of Membrane Proteins
• Toward Structural Bases for GPCR Ligand binding: A Path for Drug Discovery

Index

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