This excellent reference covers state-of-the-art methodologies for plant and animal glycoconjugate analysis--detailing mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, O-glycosylation characterization, chromophore and fluorophore labeling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.

CONTENTS

• Mass Spectrometry and NMR
• New Frontiers in Nuclear Magnetic Resonance Spectroscopy: Use of the Nano·NMR Probe for the Analysis of Microgram Quantitites of Complex Carbohydrates.
• Analysis of Anionic Glycoconjugates by Delayed Extraction.
• Matrix-Assisted Laser Desorption.
• Time-of-Flight Mass Spectrometry.
• Matrix-Assisted Laser Desorption/Ionization.
• Time-of-Flight Mass Spectrometry of Oligosaccharides Separated by High pH Anion-Exchange Chromatography.
• The Fragmentation Behavior of Glycopeptides Using the Post-Source Decay Technique and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.
• Application of Electrospray Ionization Mass Spectrometry to Unusual Phosphorylated Oligosaccharides of Trypanosomacruzi.
• Analysis of Carbohydrate Protein Interactions by Mass Spectrometry.
• Mycobacterial Lipoarabinomannans: Structural and Tagging Studies.
• Glycolipids and New Physical Methods Microscale Analysis of Glycosphingolipids by Thin-Layer Chromatography Blotting.
• High-Performance Liquid Chromatography of Gangliosides.
• A Sensitive Cell Suspension Enzyme-Linked Immunosorbent Assay Measuring the Density of Cell Surface Ganglioside and Carbohydrate Antigens.
• Scattering Techniques for the Study of Aggregative Properties of Gangliosides.
• Imaging Carbohydrate Polymers with Noncontact Mode Atomic Force Microscopy.
• Novel Approach for the Analysis of Interaction Between Sugar Chains and Carbohydrate-Recognizing Molecules Using a Biosensor Based on Surface Plasmon Resonance.
• Glycoprotein Detection Using the Light-Addressable Potentiometric Sensor.
• Protein O-Glycosylation Effects of O-Glycosylation on Protein Structure: The Role and Characterization of Heavily O-Glycosylated Mucin Domains.
• Predicting Mucin-Type O-Glycosylation Sites.
• Experimental Approaches to Studying O-Glycosylation In Vivo.
• Identifying Sites of Glycosylation in Proteins.
• High Energy Collision-Induced Dissociation of O-Linked Glycopeptides.
• Analysis of Permethylated Glycoprotein Oligosaccharide Fractions by Gas Chromatography and Gas ChromatographyMass Spectrometry.
• Labeling Oligosaccharides with Chromophores and Fluorophores.
• Highly Sensitive Precolumn Derivatization Procedures for Quantitative Determination of Monosaccharides, Sialic Acids, and Amino Sugar Alcohols of Glycoproteins by Reversed Phase High-Performance Liquid Chromatography.
• Glycan Labeling with the Fluorophores 2-Aminobenzamide and Anthranilic Acid.
• Capillary Gel Electrophoresis of 8-Aminopyrene-3,6,8-Trisulfonate-Labeled Oligosaccharides.
• Evaluation of Glycosylpyrazole Derivatives in Carbohydrate Analysis. Strategies for Oligosaccharide Separations and Sequencing.
• Discovery and Uses of Novel Glycosidases.
• Resolution of Oligosaccharides in Capillary Electrophoresis.
• A Comparison of Oligosaccharide Profiling Methods.
• High-Performance Liquid Chromatographic Mapping of OligosaccharidesUsing High pH Anion- Exchange Chromatography: Improvements from Sample Preparation, Reduction, and Fluorometric Detection.
• Monosaccharide Analysis of Recombinant Glycoproteins by High pH Anion-Exchange Chromatography/High-Performance Liquid Chromatography Using a Retractive Index Detector.
• 2-Aminobenzamide Labeling of Desialylated Oligosaccharides: A Sensitive Method for Monitoring Lot-to-Lot Consistency of Recombinant Glycoproteins.
• Characterization of Subnanomolar Amounts of N-Glycans by 2-Aminobenzamide Labeling, Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, and Computer-Assisted Sequence Analysis.
• Isolation and Characterization of N-Linked Oligosaccharides from Yeast Glycoproteins.
• High Resolution Electrophoretic Analysis of Carbohydrates Using a DNA Sequencer.
• Plant Glycobiology.
• Recent Developments in Starch Structure Analysis Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection.
• Red Seaweed Galactans: Methodology for the Structural Determination of Corallinan, a Different Agaroid.
• Some New Methods to Study Plant Polyuronic Acids and Their Esters.
• Analysis of Red Algal Extracellular Matrix Polysaccharides: Cellulose and Carrageenan.

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