Thiamine brings together the most recent developments in thiamine diphosphate (TDP)-requiring enzyme research and details the mechanisms of catalysis and structure-function relationships.

Features:

• Provides new features of the structure and mechanisms in yeast pyruvate decarboxylase
• Discusses new perspectives on the cellular role of thiamine triphosphate and thiamine triphosphatase
• Covers physiological effects of replacing the PDH complex of E. coli by genetically engineered variants or by pyruvate oxidase
• Presents new insights into neurogenerative diseases

Contents

1. Chemical Intermediates in Catalysis by Thiamine Diphosphate
2. Mechanistic and Structural Studies on Thiamine Biosynthetic Enzymes
3. Studies on the Structure and Function of Thiamine Pyrophosphokinase
4. New Perspectives on the Cellular Role of Thiamine Triphosphate and Thiamine Triphosphatase
5. How Thiamine Works in Enzymes - Time-Resolved NMR Snapshots of ThDP-Dependent Enzymes in Action
6. Thiamine-Dependent Enzymes as Catalysts of C-C-Bonding Reactions
7. The Role of Orphan Enzymes
8. Ligand-Induced Conformational Changes in Thiamine Diphosphate-Dependent Enzymes
9. Comparison Between Crystal and Solution Structures
10. Enantioselective Synthesis of Hydroxy Ketones via Benzaldehyde Lyase- and Benzoylformate Decarboxylase-Catalyzed C-C Bond Formation
11. Benzoylformate Decarboxylase - Lessons in Enzymology
12. New Concept on the Nature of the Induced Absorption Band of Holotransketolase
13. Structure of the a-Carbanion/Enamine Reaction Intermediate in the Active Site of Transketolase
14. Determined by Kinetic Crystallography
15. Yeast Pyruvate Decarboxylase - New Features of the Structure and Mechanisms
16. Solvent and Carbon Kinetic Isotope Effects on Active-Site and Regulatory
17. Site Variants of Yeast Pyruvate Decarboxylase
18. Insights into the Mechanism and Regulation of Bacterial Acetohydroxyacid Synthases
19. Structure and Properties of Acetohydroxyacid Synthase
20. Benzoylformate Decarboxylase - Intermediates, Transition States, and Diversions,
21. Structural and Functional Organization of Pyruvate Dehydrogenase Complexes

Index

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