Features:

• General Aspects of Spectroscopy
• Molecular Biology and Data Evaluation
• Introduction to NMR of Commonly Encounter Classes of Biomolecules
• Recent Developments in Spectroscopics
• Experiments and Practical Aspects Useful for the Characterization of Protein-ligand Interactions
• Account on Strategies for Drug Development using NMR

BioNMR in Drug Research presents the theoretical background on NMR of biomolecules, plus the use of NMR techniques in determining the structures of proteins and nucleic acids. The latest methods for characterizing substance-receptor complexes are demonstrated in this book, backed by many case studies from pharmaceutical research.

With its successful mixture of basic information and application strategies, coupled with many real-life examples, BioNMR in Drug Research is an invaluable guide for both NMR spectroscopists and pharmaceutical researchers.

Contents

1. Basic Techniques
   1. Modern Methods for Expression of Proteins in Isotopically Enriched Form
   2. Structure Calculation Using Automated Techniques
   3. Achieving Better Sensitivity, Less Noise and Less Artifacts in NMR Spectra

2. NMR of Biomolecules
   4. NMR Strategies for Protein Assignment
   5. NMR of Membrane-Associated Peptides and Proteins
   6. NMR of Nucleic Acids

3. Modern Spectroscopic Techniques
   7. Methods for the Measurement of Angle Restraints from Scalar and Dipolar Couplings as well as from Cross Correlated Relaxation: Application to Biomolecules
   8. Orientational Restraints
   9. Scalar Coupling Across Hydrogen Bonds
   10. TROSY:Transverse Relaxation-Optimized Spectroscopy
   11. MAS Solid-State NMR of Isotopically Enriched Biological Samples
   12. Determination of Protein Dynamics Using 15N Relaxation Measurements

4. Tools for Investigation of Drugs-Receptor Complexes and for Lingand Screening
   13. The Determination of Equilibrium Dissociation Constants of Protein-Ligand Complexes by NMR
   14. Experiments in NMR-based Screening
   15. The Use of Spin-Labels in NMR-supported Lead Finding and Optimization
   16. NMR of Weakly Binding Ligands
   17. Isotope Filter and Editing Techniques

5. Strategies for Drug Development Using NMR
   18. Strategies for NMR Screening and Library Design
   19. Strategies for Hit Finding Using NMR
   20. Strategies for Drug Discovery Using NMR
   21. NMR-based Drug Design: Approaches for Very Large Proteins

Subject Index

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