by Hans Bisswanger

Providing a broad experimental, this book contains a theoretical introduction together with practical protocols, considering all aspects of enzymology. The fundamental experiments in enzymology are presented in very clear and easily realizable protocols, with each experiment accompanied by a section giving the theoretical background.

Practical Enzymology is essential reading for all scientists and practitioners working with enzymes.

Contents

1. Introduction.
2. Enzyme Nomenclature
3. Enzyme Reactions
   • Theory of the Enzyme Reaction
   • Michaelis-Menten Equation
   • Theory of the Enzyme Assay
   • Theory of Coupled Enzyme Reactions
   • Substrate Determination
   • Instrumental Aspects
   • Enzyme Assays
   • Diverse Methods
   • Enzyme Immunoassays

4. Binding Measurements
   • Irreversible, Reversible, Specific and Unspecific Binding
   • Binding Measurements by Size Discrimination
   • Spectroscopic Techniques
   • Other Methods for Binding Measurements

5. Enzymes in Technical Applications
   • Principles of Enzyme Immobilization
   • Methods for Enzyme Immobilization
   • Analysis of Immobilized Enzymes
   • Enzyme Reactors
   • Biosensors
   • Immobilized Enzymes in Therapy

Index

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