Practical HPLC Method Development

Second Edition
by Lloyd R. Snyder et al

Thoroughly revised and greatly expanded, this Second Edition brings scientists completely up to date on method development for HPLC, one of the main analytical techniques in use today.

It offers the most far-reaching treatment available, taking into account advances in chromatography and the increasingly complex sample types that have become significant in the past decade.

This cutting-edge reference now includes:

• Comprehensive coverage of all of the essential steps of method development
• Increased focus on specialized applications for separating such newly important analytes as biological samples, mixtures of enantiomers, and preparative separations
• A complete, accessible review of HPLC separation fundamentals
• New and enhanced discussions of computer-assisted method development, HPLC column technology, and biochemical and chiral applications
• A new, more efficient approach for developing reversed-phase HPLC methods
• Exhaustive indexing and cross-referencing, enabling quick access to analysis procedures for different kinds of samples

The result of years of pathbreaking laboratory research, this invaluable reference stands at the forefront of chemical analysis.

Contents

1. Getting Started.
2. Basics of Separation.
3. Detection Sensitivity and Selectivity.
4. Sample Preparation.
5. The Column.
6. Non-Ionic Samples: Reversed- and Normal-Phase HPLC.
7. Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.
8. Gradient Elution.
9. Systematic Approach to the Reversed-Phase Separation of Regular Samples.
10. Computer-Assisted Method Development.
11. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
12. Chiral Separations.
13. Preparative HPLC Separation.
14. Quantitation (Including Trace Analysis).
15. Completing the Method: Validation and Transfer

Appendices
Index

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